产品概述:
储存条件
4℃密封避光保存,短期可放置于室温。有效期见外包装。
产品内容
组分
规格
S6009S(10mL)
S6009M(100mL)
S6009L(500mL)
A液
5 mL
50 mL
250 mL
B液
5 mL
50 mL
250 mL
Super ECL Plus 免疫印迹底物是增强型化学发光(ECL)过氧化物酶的底物,可帮助用户在免疫印迹分析过程中实现低表达或高价值蛋白的检测。
Super ECL Plus 底物可为使用辣根过氧化物酶(HRP)偶联物的免疫印迹实验提供明亮的信号,对低表达或高价值蛋白检测灵敏度很高。该 ECL 底物能够兼容各种膜、封闭液和宽范围抗体稀释液,以出色性能、通用性和高性价比,满足用户的免疫印迹应用需求。
产品特点
1. 具有极高灵敏度:检测硝化纤维素膜或 PVDF 膜上低表达或高价值的蛋白条带;2. 长信号持续时间:条件优化的情况下,经底物孵育的印迹条带能够持续输出 6 至 8 h 的可检测光信号;
3. 价格经济:配方经过优化,适用于浓度极低的抗体检测。• 0.2-1 μg/mL 一抗(或 1 mg/mL 的稀释 1:1,000-1:5,000) • 10-50 ng/mL 二抗(或 1 mg/mL 的稀释 1:20,000-1:100,000)
注意事项
1. 发光液曝露于强光下时间过久灵敏度可能有降低,操作时注意避光。戴手套可以避免在膜上留下手印。
2. 长时间曝光会加深背景;蛋白过量,会使条带强弱变化失去线性关系;曝光不足,则条带模糊或较浅。
3. 如果曝光后条带不佳,可用洗膜缓冲液洗膜,重新孵育二抗,然后重新用 ECL 曝光。
4. 使用肉眼可见的预染色蛋白 Marker 和荧光-放射自显影曝光标签可精确确定胶片上条带的位置和大小。
5. NaN3 会抑制 HRP 活性,回收二抗应避免使用 NaN3,如必需使用勿超过 0.01%。
使用本产品的文献:
1.CtIP promotes G2/M arrest in etoposide‐treated HCT116 cells in a p53‐independent manner
Hongyu Chen,Jin Shan,Dandan Chen,Ruoxi Wang,Wenjing Qi,Hailong Wang,YUEshuang Ke,Wenguang Liu,Xianlu Zeng
Journal of cellular physiology(2018)
2.Meningitic Escherichia coli-induced upregulation of PDGF-B and ICAM-1 aggravates blood-brain barrier disruption and neuroinflammatory response
Rui-Cheng Yang,Xin-Yi Qu,Si-Yu Xiao,Liang Li,Bo-Jie Xu,Ji-Yang Fu,Yu-Jin Lv,Nouman Amjad,Chen Tan,Kwang Sik Kim,Huan-Chun Chen andXiang-Ru WangJournal of Neuroinflammation(2019 16:101)
3.Melatonin enhances thrombopoiesis through ERK and Akt activation orchestrated by dual adaptor for phosphotyrosine and 3‐phosphoinositides
Shilei Chen,Yan Qi,Song Wang,Yang Xu,Mingqiang Shen,Mengjia Hu,Changhong Du,Fang Chen,Mo Chen,Yukai Lu,Zihao Zhang,Yong Quan,Cheng Wang,Fengchao Wang,Junping Wang
J Pineal Res
4.LncRNAs activate longevity regulation pathway due to aging of Leydig cells caused by DEHP exposure: A transcriptome-based study
Yuhao Wu,Junke Wang,Tianxin Zhao,Yuexin Wei,Lindong Han,Lianju Shen,Chunlan Long,Shengde Wu,Guanghui Wei
Ecotoxicology and Environmental Safety
5.Propionate and butyrate produced by gut microbiota after probiotic supplementation attenuate lung metastasis of melanoma cells in mice
Lili Chen,Xinyu Zhou,Yawei Wang,Dake Wang,Yueshuang Ke,Xianlu Zeng
Molecular Nutrition & Food Research
6.Orientin mediates protection against MRSA-induced pneumonia by inhibiting Sortase A
Li Wang , Shisong Jing, Han Qu, Kai Wang, Yajing Jin, Ying Ding, Lin Yang, Hangqian Yu , Yan Shi , Qianxue Li , Dacheng Wang
Virlence Volime 12
7.Single-Base Resolution Mapping Reveals Distinct 5-Formylcytidine in Saccharomyces cerevisiae mRNAs
Yafen Wang, Zonggui Chen, Xiong Zhang, Xiaocheng Weng, Jikai Deng, Wei Yang, Fan Wu, Shaoqing Han, Chao Xia, Yu Zhou, Yu Chen, Xiang Zhou
ACS Chemical Biology
Junchao Zhu 1, Jinghong Yuan 1, Shahrzad Arya 2, Zhi Du 1, Xijuan Liu 3, Jingyu Jia