产品概述:
产品参数
外观:可溶于 DMSO、DMF 的红色固体
Ex/Em:490/523 nm(in MeOH)
CAS 号:201860-17-5
分子式:C34H28Cl5N3O
分子量:671.9
分子结构图:
储存条件
-20℃避光保存,有效期见外包装。
产品介绍
MitoSceneTM Green I 是一种线粒体绿色荧光染料,可在纳摩尔水平对线粒体进行染色标记,该染料具有质膜透性,且在线粒体膜上积累而呈现明亮的荧光。该染料在线粒体中的定位不依赖于线粒体膜电位,染色固定细胞信噪比不理想,在细胞固定、透化之后,会导致荧光信号减弱或丢失,所以我们推荐只用来染活细胞。
注意事项
1. 荧光染料均存在淬灭问题,请尽量注意避光,以减缓荧光淬灭。
2. 为了您的安全和健康,请穿实验服并戴一次性手套操作。
使用本产品的文献:
1.Circadian clock gene NPAS2 promotes reprogramming of glucose metabolism in hepatocellular carcinoma cells
PengYuana,TaoYang,JiaoMu,JingZhao,YiYang,ZhaoyongYan,YiranHou,ChengChen,JinliangXing,HongxinZhang,JibinLi
Cancer Letters Volume 469, 28 January 2020, Pages 498-509
2. Transcriptional characteristics and functional validation of three monocyte subsets during aging.
Chen Wang, Yating Cheng , Boyu Li , Xueping Qiu , Hui Hu , Xiaokang Zhang , Zhibing Lu and Fang Zheng
Wang et al. Immunity & Ageing (2023) 20:50
参考文献
1.Staining of mitochondrial membranes with 10-nonyl acridine orange, MitoFluor Green, and MitoTracker Green is affected by mitochondrial membrane potential altering drugs.
应用方向:分析HL60细胞线粒体膜电位
2.Efficacy of MitoTracker Green and CMXrosamine to measure changes in mitochondrial membrane potentials in living cells and tissUEs.
应用方向:对线粒体特异染色,线粒体形态和功能的研究
3.MitoTracker Green labeling of mitochondrial proteins and their subseqUEnt analysis by capillary electrophoresis with laser-induced fluorescence detection.
应用方向:标记线粒体是通过与线粒体的蛋白特异结合
4.Mitotracker green is a P-glycoprotein substrate.
应用方向:染料是一种P-糖蛋白底物
5.Alveolar macrophages from subjects with chronic obstructive pulmonary disease are deficient in their ability to phagocytose apoptotic airway epithelial cells.
应用方向:追踪16HBE气道上皮细胞的有丝分裂