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  • PMA(叠氮溴化丙锭), 20 mM in water

    产品货号: P4051

    产品规格: 100 μL

    目录价(元):1061

    大包装询价


产品概述:

产品介绍

PMA是一种高亲和性的dsDNA结合染料,该染料本身具有微弱的荧光,但与核酸结合后可以发出更为明亮的荧光。PMA不具有细胞膜渗透性,因此可选择性地修饰膜受损的死细胞的 DNA。PMA修饰的DNA经光解后,PMA上的光反应性叠氮基转化为高反应性氮烯自由基,与DNA结合位点附近的任何烃部分反应形成稳定的共价氮碳键,从而导致永久性 DNA 修饰(图1),从而抑制死细胞DNA的扩增。该修饰过程还会使DNA沉淀,并使其在随后的基因组DNA提取过程中与细胞碎片一起丢失,进而阻碍死细胞中目标DNA的PCR扩增。残留在溶液中未结合的PMA,会在强光照射下与水分子反应分解成无交联活性的羟胺化合物无法共价结合DNA,从而不影响PCR扩增。PMA通过纯化去除修饰DNA 以及抑制修饰DNA扩增的组合特性使得其可以通过实时定量PCR(qPCR)的手段,检测多种样本类型包括:细菌、生物膜、酵母、真菌、病毒和真核细胞。PMA与qPCR、NGS、Sanger测序和LAMP技术相结合,可广泛应用与食品与环境安全监测领域。

图1. PMA修饰DNA后通过qPCR定量活菌和死菌的原理

应用范围

食品/环境中有害菌或者致病菌的定量检测


产品货号

P4051


储运条件

-20℃避光保存,有效期见外包装。冰袋运输。


产品参数

外观:橙红色液体

Ex (pH 3) = 464 nm (before photolysis)

Ex /Em (after photocrosslinking to nucleic acid) = 510/610 nm

分子式:C27H32Cl2N6

分子量:511.5


注意事项

1. 本产品属于荧光染料,使用及保存过程中需注意避光。

2. 本产品仅限于科研,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。

3. 为了您的安全和健康,请穿实验服并戴一次性手套操作。




说明书:


 P4051    

使用本产品的文献:


参考文献
1.Detection and quantification of viable Bacillus cereus group species in milk by propidium monoazide quantitative real-time PCR
应用方向:Bacillus cereus(蜡样芽孢杆菌)

2.Persistence of Bacteroides ovatus under simulated sunlight irradiation
应用方向Bacteroides ovales(卵巢杆菌)

3.Advantageous Direct Quantification of Viable Closely Related Probiotics inPetitSuisse Cheeses under In Vitro Gastrointestinal Conditions by Propidium Monoazide - qPCR
应用方向Bifidobacterium animalis(动物双歧杆菌)

4.Assessing the diagnostic importance of nonviable bacterial cells in respiratory infections
应用方向Burkholderia cepacia(赤胸鲨)

5.Impact of oxidative stress defense on bacterial survival and morphological change in Campylobacter jejuni under aerobic conditions
应用方向Campylobacter jejuni(空肠弯曲菌)

6.Viability-PCR Shows That NAAT Detects a High Proportion of DNA from Non-Viable Chlamydia trachomatis
应用方向Chlamydia trachomatis(沙眼衣原体 )

7.Digestibility and prebiotic properties of potato rhamnogalacturonan I polysaccharide and its galactose-rich oligosaccharides/oligomers
应用方向Clostridium leptum(瘦肉梭菌)

8. Survival and persistence of host-associated Bacteroidales cells and DNA in comparison with Escherichia coli and Enterococcus in freshwater sediments as quantified by PMA-qPCR and qPCR
应用方向Enterrococcus spp(肠球菌属)

9.Detection of viable Escherichia coli O157:H7 in ground beef by propidium monoazide real-time PCR
应用方向Escherichia coli(大肠杆菌)

10.Viability determination of Helicobacter pylori using propidium monoazide quantitative PCR
应用方向Helicobacter pylori(幽门螺杆菌)

11.Probiotic bacteria survive in Cheddar cheese and modify populations of other lactic acid bacteria
应用方向Lactobacillus acidophilus(嗜酸乳杆菌)

12.Fate of Viable but Nonculturable Listeria monocytogenes in Pig Manure Microcosms
应用方向Listeria monocytogenes(单球菌)

13.A multicenter study of viable PCR using propidium monoazide to detect Legionella in water samples
应用方向Legionella pneumophila(嗜肺军团菌)

14.Propidium monoazide and Xpert MTB/RIF to quantify Mycobacterium tuberculosis cells
应用方向Mycobacterium tuberculosis(结核分枝杆菌)

15.Determination of the effects of medium composition on the monochloramine disinfection kinetics of Nitrosomonas europaea by the propidium monoazide quantitative PCR and Live/Dead BacLight methods
应用方向Nitrosomonas europae(欧洲硝酸铵)

16.Study and Understanding Behavior of Alginate-Inulin Synbiotics Beads for Protection and Delivery of AntimicrobialProducing Probiotics in Colonic Simulated Conditions
应用方向Pediococcus acidilactici(酸乳球菌)

17.Quantification of Pseudomonas aeruginosa in multispecies biofilms using PMA-qPCR
应用方向Pseudomonas aeruginosa(铜绿假单胞菌)

18.Multiplex PCR coupled with propidium monoazide for the detection of viable Cronobacter sakazakii, Bacillus cereus, and Salmonella spp. in milk and milk products
应用方向Salmonella enterica(肠炎沙门菌)

19.Peptoid Efficacy against Polymicrobial Biofilms Determined by Using Propidium Monoazide-Modified Quantitative PCR
应用方向Staphylococcus aureus(金黄色葡萄球菌)

20.Method to quantify live and dead cells in multi-species oral biofilm by real-time PCR with propidium monoazide
应用方向Streptococcus oralis(口腔链球菌)

21. OxyRactivated expression of Dps is important for Vibrio cholerae oxidative stress resistance and pathogenesis
应用方向Vibrio cholerae(霍乱弧菌)

22. A New Physiological Role for the DNA Molecule as a Protector against Drying Stress in Desiccation-Tolerant Microorganisms
应用方向Microbacterium 3J1(微杆菌3J1)

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